Cameron House on Loch Lomond has been put up for sale separately from the rest of the QHotels portfolio.
The five-AA-star, 132-bedroom hotels was one of six properties within De Vere Hotels which was sold to Sankaty Advisors and Canyon Capital Advisors, the owners of QHotels, in November 2014 for around £160m.
Sankaty and Canyon, which started proceedings to offload the 27-strong QHotels group last month, now intends to sell Cameron House as a separate entity.
The sale of the majority of QHotels is being handled by Rothschilds, who is looking to achieve a figure of around £650m, while CBRE is selling Cameron House for a rumoured £80m.
Cameron House, which comprises two golf courses , a spa and 184 timeshare lodges, is the second major resort hotel in Scotland to come to market in recent months. The five-AA-red-star, 232-bedroom Gleneagles resort, host venue of the 2014 Ryder Cup, is currently for sale for more than £200m.
Other hotels within the QHotels group includes the four-AA-star, 312-bedroom Midland hotel in Manchester and the four-AA-star, 215-bedroom Queens in Leeds.