The investment is set to enable Redefine BDL Hotels, which currently operates 54 branded and unbranded hotels with a turnover in excess of £200m, to grow its portfolio.
Tsogo's shareholding in the company will sit alongside the 25.3% proportion owned by Redefine International, a UK real estate investment trust.
Helder Pereira, chief executive of Redefine BDL Hotels, said the company is excited by the opportunities that lie ahead, suggesting that the investment will bolster the potential for growth at "an accelerated rate".
"Our experience, scale and consistent track record coupled with the credentials of Tsogo Sun Holdings will ensure our reputation as a major player in the hotel management sector."
Marcel von Aulock, chief executive at Tsogo Sun, added: "There is the potential for opportunities to deploy capital in attractive investments in the European market and the investment in Redefine BDl Hotels gives an ideal platform to achieve this."
As well as managing independent hotels, Redefine BDL also operates hotels under brands belonging to Hilton Worldwide, Best Western, InterContinental Hotels Group, Wyndham Hotels & Resorts and Starwood Hotels & Resorts.