Travelodge is investigating how a threat of rape was posted on the television screen in a bedroom of the budget brand's Whitemare Pool hotel, near Gateshead, Tyne & Wear.
A female guest found the message, "I'm going to rape you", when she checked in to her room.
A spokesperson for Travelodge said the company had apologised that such a message could appear.
"We take the security of our guests and our systems very seriously and while this is an isolated incident, applicable to only one room, we have consequently launched a full investigation with our television supplier.
"We will take the appropriate action to address any issues that are identified by our investigation and in the interim, we have reaffirmed our guidance to team-members regarding checks on the status of televisions in our guest-rooms."