London nightclub Fabric has announced it will appeal against the Council's decision to revoke its licence.
Islington Council revoked the club's licence on Wednesday due to concerns about drugs use.
Earlier this year, Fabric was forced to close when two teenagers died from suspected drug overdoses at the club, which is located in Farringdon.
A statement on Fabric's website says: "We are extremely disappointed with Islington Council's decision to revoke our licence. This is an especially sad day for those who have supported us, particularly the 250 staff who will now lose their jobs. Closing Fabric is not the answer to the drug-related problems clubs like ours are working to prevent, and sets a troubling precedent for the future of London's night time economy."
A #savefabric campaign was set up as an attempt to stop the council's decision. It has been signed by almost 160,000 people.
Jacob Husley, a promoter and DJ for Fabric, set up the petition. He said: "Fabric does its best to keep customers safe and protect them from drugs and adopt a zero drugs policy. Closing a venue such as Fabric would be an incredibly short-sighted response to a complex problem."
The Night Time Industries Association is backing the campaign and hopes to raise £500,000 to help the club legally fight the council's decision.
The decision to revoke the licence was met with criticism from London's Mayor Sadiq Khan. He said: "London's iconic clubs are an essential part of our cultural landscape. As mayor, I'm determined to do more to protect them. Fabric, the Metropolitan Police and Islington will of course have to take real action to protect the safety of everyone who enjoys a night out at the club.
"My team have spoken to all involved in the current situation and I am urging them to find a common sense solution that ensures the club remains open while protecting the safety of those who want to enjoy London's clubbing scene.