Stonegate Pub Company has revealed "up to 260 roles" could be made redundant as it continues a "strategic review of the organisation".
It comes after the group reported that it was looking to sell around 1,000 of its pubs in February.
As part of the strategic review, which started a few months ago, the owner of the Be At One and Slug and Lettuce brands will restructure its "central functions and operations management teams".
A spokesperson for Stonegate Pub Company said: "Regrettably, this means that we will enter into a consultation process on proposals which may result in up to 260 roles being made redundant. These are difficult decisions to take, and our priority over the coming weeks will be to consult with affected colleagues and to continue to look after their wellbeing.
"Wherever possible, we will avoid redundancies by finding people alternative roles and will ensure that those colleagues who are made redundant are fully supported by the business."
The spokesperson added: "The group is confident this strategy will best position us to deliver profitable growth."
Stonegate is owned by private equity firm TDR Capital and became the largest pub company in the UK when it acquired rival chain Ei Group in 2020, taking it to around 4,500 sites.