The Bishop's Mill in Durham, the Blacksmith's Forge in Dalkeith, Scotland Yr Hen Orsaf in Aberystwyth, Wales, and the Old Court House in Coleraine, Northern Ireland, were all singled out for their cleanliness.
More than 80 Wetherspoon pubs were recognised for their excellent standards by inspectors who made surprise visits to hundreds of pubs over a three-month period.
Loo of the Year awards director Richard Chisnell said: "The toilets are judged against more than 100 different inspection criteria, including decor and maintenance, cleanliness, accessibility, hygiene equipment and overall management.
"Wetherspoon is a worthy winner of the four main individual pub awards and the company continues to be the best pub toilet provider in the UK."
John Hutson, chief executive at Wetherspoon, said: "We are delighted with the awards and it highlights our commitment to providing quality toilets at all of our pubs."
By Christopher Walton
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